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M94A3164.TXT
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1994-10-25
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Document 3164
DOCN M94A3164
TI A study of viral load in HIV-2 infection in a rural community of west
Africa.
DT 9412
AU Ariyoshi K; Berry N; Wilkins A; Ngom PT; Tedder RS; Whittle H; M.R.C.
Lab., Gambia, West Africa.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0174). Unique
Identifier : AIDSLINE ICA10/94369411
AB OBJECTIVES: To determine HIV-2 proviral load in seropositive individuals
in a rural village in Guinea-Bissau and to investigate the effects of
aging and co-infection with HTLV-I, malaria and syphilis. METHODS: DNA
samples from 88 subjects were investigated for quantitative PCR with
utilising HIV-2 LTR primers and the external standard control made by
CBL-22 infected C8166 cell line. Biotinylated primer were used to
capture the 35S dATP incorporated secondary PCR product in a
quantitative radiometric assay. Sera were tested for TPHA, RPR and
HTLV-I (Fujirebio). HTLV infection was further examined by a nested PCR
with tax/rex primers and Taq1/Sau3a enzyme analysis of the product.
RESULTS: 81/88 samples were positive by HIV-2 nested PCR (sensitivity =
92%). The mean of HIV-2 proviral copy number (log10) per 10(5) CD4+
cells in a group with low CD4+ counts (< 200) was 16-fold higher than in
a group with high CD4+ counts (> or = 500) (mean +/- S.D. 3.15 +/- 0.71
and 1.95 +/- 1.15 respectively). 25 individuals were found to be
co-infected with HTLV-I by PCR and the enzyme analysis. Concordant rate
between HTLV serology and PCR was 82%. CD4% was significantly higher in
individuals with HTLV co-infection (p < 0.05) nonetheless HIV-2 viral
load tends to be higher in HTLV co-infected individuals especially in
the elderly (> or = 60 years) (mean +/- S.D. 2.47 +/- 1.37 vs 1.37 +/-
1.08). The viral load was lower in the HTLV ve elderly than in the
young. No significant effect on viral load was seen with malaria or
syphilis. CONCLUSIONS: The result shows a possible enhancing effect of
HTLV-I co-infection on HIV-2 viral load despite the CD4% being higher.
An inverse association between HIV-2 viral load and age was seen,
suggesting that long-term survival may be associated with low viral
load.
DE Acquired Immunodeficiency Syndrome/COMPLICATIONS/DIAGNOSIS/
*MICROBIOLOGY Age Factors Aging DNA Primers Genes, pX Guinea-Bissau
Human HIV Long Terminal Repeat HIV-2/GENETICS/*ISOLATION & PURIF
HTLV-I Infections/COMPLICATIONS/DIAGNOSIS
Malaria/COMPLICATIONS/DIAGNOSIS Polymerase Chain Reaction/*METHODS
Proviruses/GENETICS/ISOLATION & PURIF Restriction Mapping Rural
Population Sensitivity and Specificity
Syphilis/COMPLICATIONS/DIAGNOSIS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).